The Second Menzies Ministry was the twenty-seventh Australian Commonwealth ministry, and ran from 14 March 1940 to 28 October 1940.

United Australia Party–Australian Country Party Coalition

• Rt Hon Robert Menzies, KC MP: Prime Minister, Minister for Defence Co-ordination, Minister for Information. Minister for Munitions (from 11 June 1940) (UAP)
• Hon Archie Cameron, MP: Minister for Commerce, Minister for the Navy (CP)
• Rt Hon Billy Hughes, KC MP: Attorney-General, Minister for Industry (UAP)
• Hon Geoffrey Street, MP: Minister for the Army, Minister for Repatriation (to 13 August 1940) (UAP)
• Hon Sir Henry Gullett, KCMG MP: Vice-President of the Executive Council, Minister in charge of Scientific and Industrial Research (to 13 August 1940) (UAP)
• Senator Hon George McLeay: Minister for Trade and Customs (UAP)
• Hon James Fairbairn, MP: Minister for Civil Aviation, Minister for Air (to 13 August 1940) (UAP)
• Hon Sir Frederick Stewart, MP: Minister for Social Services, Minister for Supply and Development (UAP)
• Senator Hon Harry Foll: Minister for the Interior (UAP)
• Senator Hon Herbert Collett: Minister without portfolio in charge of War Service Homes, Minister without portfolio assisting the Minister for Repatriation (to 14 August 1940). Minister in charge of War Service Homes, Vice-President of the Executive Council, Minister in charge of Scientific and Industrial Research (from 14 August 1940). (UAP)
• Senator Hon Philip McBride: Minister without portfolio assisting the Minister for the Minister for Commerce (to 14 August 1940), Minister for the Army, Minister for Repatriation (from 14 August 1940) (UAP)
• Hon Percy Spender, KC MP: Treasurer (UAP)
• Hon John McEwen, MP: Minister for External Affairs (CP)
• Hon Harold Thorby, MP: Minister for Health, Postmaster-General (CP)
• Hon Arthur Fadden, MP: Minister without portfolio assisting the Minister for Supply and Development, Minister without portfolio assisting the Treasurer (CP) (to 14 August 1940). Minister for Air, Minister for Civil Aviation, Minister assisting the Treasurer (from 14 August 1940) (CP)
• Hon Horace Nock, MP: Minister without portfolio assisting the Prime Minister, Minister without portfolio in charge of External Territories, Minister without portfolio assisting the Minister for the Interior (CP)