The First Holt Ministry was the forty-second Australian Commonwealth ministry, and ran from 26 January 1966 to 14 December 1966.

Liberal Party of Australia–Australian Country Party Coalition

Cabinet

• Rt Hon Harold Holt, CH MP: Prime Minister
• Rt Hon John McEwen, MP: Minister for Trade and Industry (CP)
• Hon William McMahon, MP: Treasurer
• Hon Paul Hasluck, MP: Minister for External Affairs
• Hon Charles Adermann, MP: Minister for Primary Industry (CP)
• Hon Allen Fairhall, MP: Minister for Defence
• Senator Hon Denham Henty: Minister for Supply
• Hon Alan Hulme, MP: Postmaster-General, Vice-President of the Executive Council
• Hon David Fairbairn, MP: Minister for National Development
• Hon Charles Barnes, MP: Minister for Territories (CP)
• Senator Hon John Gorton: Minister for Works, Minister in charge of Commonwealth Activities in Education and Research under the Prime Minister
• Hon Leslie Bury, MP: Minister for Labour and National Service

Junior ministry

• Hon Gordon Freeth, MP: Minister for Shipping and Transport
• Hon Reginald Swartz, MBE (Mil) MP: Minister for Civil Aviation
• Hon Hubert Opperman, MP: Minister for Immigration
• Hon Billy Snedden, QC MP: Attorney-General
• Hon Dr James Forbes, MP: Minister for Health
• Hon Doug Anthony, MP: Minister for the Interior (CP)
• Hon Fred Chaney, MP: Minister for the Navy
• Hon Peter Howson, MP: Minister for Air, Minister assisting the Treasurer
• Senator Hon Ken Anderson: Minister for Customs and Excise
• Senator Hon Colin McKellar: Minister for Repatriation (CP)
• Hon Ian Sinclair, MP: Minister for Social Services (CP)
• Senator Hon Dame Annabelle Rankin, DBE: Minister for Housing
• Hon Malcolm Fraser, MP: Minister for the Army